Individual laboratories are equipped with modern instrumentation for biophysical investigations. The resources include X-ray diffractometers for the determination of three-dimensional structure of biomolecules; microcalorimetry equipment for thermodynamic analyses of proteins and their affinity for other molecules; state-of-the-art fluorescence and circular dichroism spectrophotometers for characterizing protein and nucleic acid folding; and advanced computing power for database analysis and various structural and dynamic calculations.
In addition, multiple facilities are available to PMB participants through the Johns Hopkins University and Medical Institutions.
CMB provides the resources and intellectual environment to facilitate biophysical research, both for biophysically oriented laboratories, and for those laboratories whose research has not traditionally involved structural or biophysical characterization.
Accurate mass measurements for small and large molecules can be obtained at the Mass Spectrometry Facility of the Chemistry Department and the Proteomics Facility of the Institute for Basic Biomedical Sciences.
A structural analysis center in the New Chemistry Building on the Homewood campus.
Located in the Department of Biophysics and Biophysical Chemistry at the School of Medicine, this facility is fully equipped with several X-ray generators, detectors, and computers. It is maintained as a departmental facility and staffed by a full-time manager.
State-of-the-art solution state NMR equipment is available at the Biomolecular NMR Center on the Homewood campus and at the School of Medicine NMR Facility. The instruments include 500, 600, and 800 MHz-NMR spectrometers. In-depth training is provided by Dr. Ananya Majumdar, director of the center.
Protein and nucleic acids can be made or analyzed with excellent turn-around time at the Synthesis and Sequencing Facility of the School of Medicine.
Also available to the Hopkins community:
The Biophysics Core Facility supports Johns Hopkins Malaria Research Institute investigators who want to characterize macromolecules or macromolecular complexes using biophysical techniques (isothermal titration calorimetry, dynamic light scattering, circular dichroism, and x-ray crystallography).
Instrumentation necessary for basic and advanced light and electron microscopy (LM/EM), including 3-D and 4-D imaging, FRET, FRAP, and FCS, among others.
Compound repository and high throughput robotics laboratory services for enzymatic and biologic assays.
For the study of interactions of chemical compounds, gene products, cells, and organisms, and the networks formed by these interactions.
For the characterization and separation of particles as diverse as beads, cells, and chromosomes.
For light and electron microscopy and electronic imaging.
Department of Chemistry, School of Arts and Sciences
The Chemistry Department has a wide array of instrumentation in addition to the NMR and spectroscopy facilities mentioned above.
Institute for Basic Biomedical Sciences Technology Hubs, School of Medicine
In addition to those listed above are the Genetic Resources Core Facility, Microarray Facility, Middle Atlantic Mass Spectrometry Laboratory, and Protein/Peptide Sequencing Facility.
Johns Hopkins Malaria Institute Core Facilities, School of Public Health
In addition to the Biophysics Core facility, the Institute has an Imaging and Microscopy Core, and a Gene Array Core.